A collection of 81 plugs were obtained from the Humilly-2 borehole (France), that reached the Permo-Carboniferous sediments at a depth of 3051 m. Experimental measurements of physical parameters and mineralogical analysis were performed to explore the links between sedimentary facies and seismic characteristics and provide a key tool in the interpretation of seismic field data in terms of geological formations. The plugs, cylinders of 22.5 mm in diameter and ∼30mminlengthwerecollectedparallelandperpendiculartothebeddinginordertoexploretheiranisotropy.Ultrasoundwavepropagationwasmeasuredatincreasingconfiningpressureconditionsupto260MPa,apressurewhereallmicro−fracturesareconsideredclosed.Thederivativesofvelocitieswithpressurewereestablished,allowingthesimulationoflithologicaltransitionsatin−situconditions.Atroomconditions,measuredgraindensities(kg/m3)rangefrom2630to2948andvelocitiesvaryfrom4339to6771and2460to3975m/sm/sforP−andS−wavespropagationmodes,respectively.Thelargestseismic−reflectionscoefficientswerecalculatedfortheinterfacebetweentheevaporiticfaciesoftheKeuper(Lettenkohle)andtheunderlyingMuschelkalkcarbonates(Rc=c=0.3).Theeffectiveporosityhastherangeof0.23 × 10 - 16. A positive correlation between porosity and ultrasound velocity has been observed for P- and S-waves. The link between velocities and modal content of quartz, dolomite, calcite, and micas has been explored. This paper presents a unique set of seismic parameters potentially useful for the calibration of seismic data in the Geneva Molasse Basin.